Home > Sandwich ELISA Kits > Human Mannose Associated Serine Protease 1 (MASP1) ELISA Kit

Product Name:

Human Mannose Associated Serine Protease 1 (MASP1) ELISA Kit

Cat.#:

JL16890

Regulatory Status:

RUO

Alternative:

CRARF; PRSS5; Mannan-Binding Lectin Serine Peptidase 1; Ra-reactive factor serine protease p100; Complement-activating component of Ra-reactive factor; Serine protease 5

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

93.7-6000pg/mL

Sensitivity:

46.6pg/mL

Specificity:

This kit recognizes Human MASP1 in samples. No significant cross-reactivity or interference between Human MASP1 and analogues was observed.

Sample Type:

Serum, plasma and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MASP1

Mannan-binding lectin serine protease 1 also known as mannose-associated serine protease 1 (MASP-1) is an enzyme that is encoded by the MASP1 gene.MASP-1 is involved in the lectin pathway of the complement system and is responsible for activating MASP-2 and MASP-3. It is also involved in the process of cleaving complement proteins, C4 and C2, into fragments to form a C3-convertase.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	6000	3000	1500	750	375	187.5	93.7	0
OD	2.59	1.47	0.81	0.48	0.33	0.23	0.21	0.08
Corrected OD	2.51	1.39	0.73	0.4	0.25	0.15	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MASP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Human MASP1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
1 : 2	Average (%)	91	92
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98